Biology Centre CAS

Laboratory of Electron Microscopy

Address

Biology Centre CAS
Branišovská 1160/31
370 05 České Budějovice

www.paru.cas.cz/en/sections/laboratory-of-electron-microscopy
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Contacts

Head

Marie Vancová
Phone: +420 387 775 938
E-mail: vancova@paru.cas.cz

The Laboratory of Electron Microscopy was founded at the beginning of 70th as an open workplace with the main task to provide services in the field of biological electron microscopy. At present the laboratory is a supporting facility for research groups from shared campus of the Biology Centre of Academy of Sciences of the Czech Republic and the Faculty of Sciences of the University of South Bohemia at Ceske Budejovice.

Expertise and support provided to users

The electron microscopy facility helps scientists from different fields to carry out their research using cutting-edge instrumentation and provides training and services from sample preparation to image analysis.


Imaging technologies available
Transmission electron microscopy including observation at at cryogenic temperatures, (cryo-) single particle analysis, and (cryo)- tilt-series electron tomography.
Scanning electron microscopy including observation at cryogenic temperatures (freeze-fracturing, freeze-etching), array tomography, serial block-face electron tomography.
Localizations of molecules, correlative light-electron microscopy.

Imaging technologies available

  • Localizations of molecules, correlative light-electron microscopy.
  • Scanning electron microscopy
  • Transmission electron microscopy
  • Electron Microscopy

Equipment available (technical specification)

JEOL JEM-1400

  • Accelerating voltage: 120 kV
  • Cathode: LaB6
  • Resolving power: TEM – point 0.38 nm, lattice 0.2 nm
  • Observation of cryogenic specimens by using the cryogenic holder

JEOL JEM–2100F

  • Accelerating voltage: 200 kV
  • Optional: 120 kV and 100 - 80 kV
  • Cathode: Schottky emission (W-ZrO)
  • Resolving power: TEM – point 0.27 nm, lattice 0.14 nm
  • Image capture: GATAN K2 Summit direct detector 3838x3710, Super-resolution 7676x7420, linear mode, counting mode with drift correction
  • JEOL Phase Plate (VOLTA)
  • Tomography cryo holder Elsa 698 with temperature controller
  • Cryo-transfer workstation
  • Software for automated single particle analysis imaging
  • Motorized stage with piezoelectric movement
  • SerialEM tomography software

JEOL JSM-7401F

  • Accelerating voltage: 0.1 - 30 kV
  • Cathode: Field emission (W - single crystal)
  • Resolving power: 1 nm
  • Image capture: 1280×1024×8bit (with averaging 10bit)
  • Cryo attachment: Cryo-ALTO 2000 (GATAN) allows:
  • o Sample freezing (possible transfer from HPF or Plunge Freezer)
  • o Freeze fracturing
  • o Freeze sublimation and sputter coating Au, Pt/Pd

• Vacuum: 10-7 Pa
Apreo (Thermo Fisher Scientific)
• Accelerating voltage: 0.2 – 30 kV
• Electron landing energy: 20 eV – 30 keV
• Cathode: Field emission (Schottky)
• Resolving power: 0.7nm @15kV, 0.8nm @1kV, 0.9nm @500V, STEM 0.8nm @30kV, LowVac 1.8nm @3kV
• Image capture: up to 6144 x 4096 px
• Vacuum: HiVac 10-2 – 10-4 Pa, LowVac 10–500 Pa
• Stage: high tilt range +-90 degrees
• Extension: Serial Block Face tomography (VolumeScope), Stitching software (MAPS), STEM holder for 6 TEM grids, Amira visualization software
High-pressure-freezer EM ICE and freeze-substitution unit EM AFS2 (Leica Microsystems)
Ultramicrotomes: Leica UCT 6 with cryo-attachment and cryo-manipulator; Leica UCT with cryo-attachment, Leica UCT 7
Ion sputter coater Bal-Tec SCD 050 for the deposition of gold and optionally other metals.
Critical Point Dryer Pelco
Solarus II advanced plasma system (pumping station with plasma cleaner Ar+O2)
The Enviro-Genie for the study of microorganisms and tissue cell growth under strictly controlled conditions of temperature and agitation

Research topics directly investigated in the CF

Biological specimen preparation for Volume SEM
Cathodoluminescence scanning electron microscopy for life science

External access to the equipment

https://www.paru.cas.cz/en/sections/laboratory-of-electron-microscopy

Courses

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Training materials (tutorials, handbooks etc.)

https://www.paru.cas.cz/en/sections/laboratory-of-electron-microscopy/teaching/

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