Masaryk University, CEITEC - Central European Institute of Technology

Cellular Imaging Core Facility

Address

Masaryk University, CEITEC - Central European Institute of Technology
Kamenice 753/5, Bohunice
625 00 Brno-město

www.ceitec.eu
www.ceitec.eu/cellular-imaging/cf119

Contacts

Head

Milan Ešner
Phone: 00420 54949 6133
E-mail: milan.esner@ceitec.muni.cz

Core Facility Cellular Imaging (CELLIM) provides services in the area of light microscopy and offers comprehensive expertize related to sample preparation, image acquisition and data analysis. We also provide access to widefiled and confocal microscopes and individual training of users in operation of microscopes, image acquisition and data analysis.

Expertise and support provided to users

Services and Methodologies Provided
• Access to widefiled and confocal microscopes
• Access to image analysis tools
• Project design and assistance in planning of experiments
• Training of users in sample preparation, image acquisition and data analysis
• Data acquisition and image analysis provided by CF staff

Imaging technologies available

  • Laser Scanning Confocal Microscopy (LSM / CLSM)
  • Deconvolution Widefield Microscopy
  • Fluorescence-Lifetime Imaging Microscopy (FLIM)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)

Equipment available (technical specification)

ZEISS  LSM 700 confocal microscope, ZEISS LSM 780 confocal microscope with Airyscan detector, ZEISS LSM 800 confocal microscope, ZEISS LSM 880 confocal microscope with AiryScan Fast detector, Olympus SZX16 steremicroscope, ZEISS AxioImager.Z2-ZEN widefield fluorescence microscope, ZEISS AxioImager.2-Visitron widefield fluorescence microscope, ZEISS Axioscope A1 histological microscope, Workstation1-IMARIS, Workstation2-Deconvolution.

Research topics directly investigated in the CF

4D quantitative microscopy, Live cell imaging techniques

External access to the equipment

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Courses

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Training materials (tutorials, handbooks etc.)

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