BIOCEV, Faculty of Science, Charles University

Imaging Methods Core Facility (IMCF) at BIOCEV


BIOCEV, Faculty of Science, Charles University
Průmyslová 595
25242 Vestec near Prague



Aleš Benda
Phone: +420 735 196 355

IMCF covers three main areas-optical (fluorescence) microscopy, electron microscopy and flow cytometry. We offer our users training on and access to high end instrumentation, plus we provide consulting services on optimal experimental design, sample preparation, data acquisition and analysis and interpretation of the obtained results.

Expertise and support provided to users


Imaging technologies available

  • Laser Scanning Confocal Microscopy (LSM / CLSM)
  • Deconvolution Widefield Microscopy
  • Multiphoton Microscopy Systems
  • Total Internal Reflection Fluorescence Microscopy (TIRF)
  • Stimulated Emission Depletion Microscopy (STED)
  • Photo Activated Localization Microscopy (PALM)
  • Stochastic Optical Reconstruction Microscopy (STORM)
  • Fluorescence Correlation Spectroscopy (FCS)
  • Fluorescence Cross-Correlation Spectroscopy (FCCS)
  • Fluorescence-Lifetime Imaging Microscopy (FLIM)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Electron Microscopy
  • Correlative Light Electron Microscopy (CLEM)
  • Non-Linear Microscopy Techniques (e.g. CARS, SHG, THG, SRS)
  • Single Molecule Imaging Techniques
  • Other

Equipment available (technical specification)

  1. Nikon Ti-E with N-SIM and N-STORM 3D wide-field super-resolution microscope
  2. Nikon Ti-E with easy 3D STED from Abberior Instruments confocal super-resolution microscope
  3. Carl Zeiss LSM 880 NLO spectral confocal and MP+OPO scanning microscope
  4. Leica TCS SP8 WLL SMD-FLIM+FCS confocal microscope
  5. Leica TCS SP8 DM6 CFS electro-physiological upright confocal microscope
  6. Leica DMi8 inverted wide-field electro-physiological microscope
  7. Nikon Ti-E with H-TIRF inverted wide-field microscope
  8. Carl Zeiss AxioZoom.V16 upright fluorescence macroscope
  9. LUMICKS Super/C-Trap integrated optical tweezers and confocal microscopy
  10. FEI Helios NanoLab G3 UC scanning electron microscope with focused ion beam milling
  11. Leica EM HPM100 - High-pressure freezing cryo-fixation
  12. Leica ULTRACUT EM UC7 with Leica EM FC7 Cryo Chamber with VCT - Ultramicrotome with cryo-chamber
  13. Leica EM AFS2 - Freeze Substitution and Low Temperature Embedding System
  14. Leica EM CPD300 A - Critical point drier

Research topics directly investigated in the CF


External access to the equipment




Training materials (tutorials, handbooks etc.)



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