Institute of Molecular Genetics CAS

Light Microscopy Core Facility

Address

Institute of Molecular Genetics CAS
Videnska 1083
14220 Praha

www.img.cas.cz
www.img.cas.cz/servisni-pracoviste/centrum-mikroskopie/light-microscopy-and-flow-cytometry/

Contacts

Head

Ondrej Horváth
Phone: +420 24106 3168
E-mail: ondrej.horvath@img.cas.cz

The facility provides methodological and instrumentation background for fluorescence microscopy techniques and image analysis. The facility is equipped with wide range of instruments, ranging from routine fluorescence microscopes, confocal microscopes up to high-end super-resolution microscopy systems STED, SIM and SMLM. Facility is providing the services for in-house research groups and for wider microscopy community as well, on open access principle. Most of the instrumentation in the laboratory is available on the self-service basis, for trained users. We put big emphasis on user education and training and provide personalized assistance to users and their projects, not only in the sense of sample preparation and image acquisition, but on data processing, reconstruction and analysis as well. Several offline workstations for data analysis is available (Huygens Pro, MetaMorph, Fiji - ImageJ, Helicon, MatLab, SoftWorx).

Expertise and support provided to users

sample preparation for fluorescence microscopy and super resolution microzcopy techniques, widefield, confocal and super-resolution imaging. Image processing, restoration, deconvolution and analysis.

Imaging technologies available

  • Laser Scanning Confocal Microscopy (LSM / CLSM)
  • Spinning Disc Confocal Microscopy (SDCM)
  • Deconvolution Widefield Microscopy
  • Total Internal Reflection Fluorescence Microscopy (TIRF)
  • Stimulated Emission Depletion Microscopy (STED)
  • Photo Activated Localization Microscopy (PALM)
  • Stochastic Optical Reconstruction Microscopy (STORM)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • High-Throughput Microscopy
  • Quantitative Phase Imaging (CCHM - Coherence Controlled Holographic Microscopy)
  • Selective Plane Illumination Microscopy (SPIM)
  • Other

Equipment available (technical specification)

Leica TCS SP5 AOBS Tandem Confocal microscope

Leica TCS SP8 confocal microscope

Leica DMI6000 Inverted fluorescence microscope with TIRF illumination

Inverted fluorescence microscope Delta Vision Core with laser photo-manipulation

Olympus Cell^R/Scan^R high throughput fluorescence microscope

Leica LMD6000 Laser Microdissection Microscope

Leica DM6000 fluorescence microscope

Leica DMI6000 inverted fluorescence microscope

Leica TCS SP8 STED 3X super resolution microscope

DeltaVision OMX™ with the Blaze SIM Module, DSSI localization and TIRF illumination

Zeiss Light Sheet Z.1 SPIM microscope

Axiozoom V16 with Apotome

Tescan quantitave phase microscope

Andor Dragonfly 503 spinning disk microscope with photomanipulation

Research topics directly investigated in the CF

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External access to the equipment

http://www.img.cas.cz/core-facilities/microscopy-centre/light-microscopy-and-flow-cytometry/

Courses

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Training materials (tutorials, handbooks etc.)

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News


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