Biology Centre CAS
370 05 České Budějovice
Phone: +420 387 775 938
The Laboratory of Electron Microscopy was founded at the beginning of 70th as an open workplace with the main task to provide services in the field of biological electron microscopy. At present the laboratory is a supporting facility for research groups from shared campus of the Biology Centre of Academy of Sciences of the Czech Republic and the Faculty of Sciences of the University of South Bohemia at Ceske Budejovice.
The electron microscopy facility helps scientists from different fields to carry out their research using cutting-edge instrumentation and provides training and services from sample preparation to image analysis.
Imaging technologies available
Transmission electron microscopy including observation at at cryogenic temperatures, (cryo-) single particle analysis, and (cryo)- tilt-series electron tomography.
Scanning electron microscopy including observation at cryogenic temperatures (freeze-fracturing, freeze-etching), array tomography, serial block-face electron tomography.
Localizations of molecules, correlative light-electron microscopy.
• Vacuum: 10-7 Pa
Apreo (Thermo Fisher Scientific)
• Accelerating voltage: 0.2 – 30 kV
• Electron landing energy: 20 eV – 30 keV
• Cathode: Field emission (Schottky)
• Resolving power: 0.7nm @15kV, 0.8nm @1kV, 0.9nm @500V, STEM 0.8nm @30kV, LowVac 1.8nm @3kV
• Image capture: up to 6144 x 4096 px
• Vacuum: HiVac 10-2 – 10-4 Pa, LowVac 10–500 Pa
• Stage: high tilt range +-90 degrees
• Extension: Serial Block Face tomography (VolumeScope), Stitching software (MAPS), STEM holder for 6 TEM grids, Amira visualization software
High-pressure-freezer EM ICE and freeze-substitution unit EM AFS2 (Leica Microsystems)
Ultramicrotomes: Leica UCT 6 with cryo-attachment and cryo-manipulator; Leica UCT with cryo-attachment, Leica UCT 7
Ion sputter coater Bal-Tec SCD 050 for the deposition of gold and optionally other metals.
Critical Point Dryer Pelco
Solarus II advanced plasma system (pumping station with plasma cleaner Ar+O2)
The Enviro-Genie for the study of microorganisms and tissue cell growth under strictly controlled conditions of temperature and agitation
Biological specimen preparation for Volume SEM
Cathodoluminescence scanning electron microscopy for life science
September 9, 2022 | Brno
Course | 13th – 14th June 2022 | BC, České Budějovice
Registration for conference is open.
Course | 7th – 9th June 2022 | IMG
Course | 9th – 13th May 2022 | BIOCEV
Practical Course | 15th – 26th August 2022 | Brno, Czech Republic
Course | 1st – 2nd June 2022 | CELLIM, CEITEC MU
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