Brno node

Advanced Light Microscopy and Medical Imaging

 

The Czech Advanced Light Microscopy and Medical Imaging Multi Modal Node in Brno provides open access to a wide range of imaging technologies and expertise to all scientists through a unified and coordinated logistics approach. The light microscopy and medical imaging units organize special programs for training scientists in biological and medical imaging techniques and data analysis.

Specialties and expertise of the Node

 

The Euro-BioImaging Node Candidate in Brno consists of two parts. The Medical Imaging part is formed by two closely collaborating facilities. One is focused on animal ultra-high field MR imaging and spectroscopy (9.4T), the other on human MR imaging (3T) accompanied with electrophysiological techniques. Together, these facilities enable translational research and offer a complex portfolio of MRI techniques including multimodal approaches (e.g. simultaneous EEG-fMRI) and human hyper-scanning (fMRI with two participants measured simultaneously in two scanners). The Advanced Light Microscopy part is organized as a central core facility Cellular Imaging (CELLIM) and several closely cooperating laboratories offering together access to equipment, training and image analysis tools. The light microscopy is further specialized in imaging of plant systems, mammalian germs cells, stem cells and embryos, and development of image analysis tools.

Offered Technologies:

  • Laser scanning confocal microscopy (LSCM)
  • Fluorescence correlation spectroscopy (FCS)
  • Fluorescence-lifetime imaging microscopy (FLIM)
  • Deconvolution widefield microscopy (DWM)
  • MRI for humans (two whole-body 3T scanners) accompanied with electrophysiology for simultaneous measurement in MRI
  • Ultra-high field MRI/MRS for small animals (9.4T/30cm scanner, accredited for mice/rats/rabbits)

Instrument highlights

 

The Brno Node is equipped with two 3T Siemens Prisma scanners for human medical imaging. These scanners are designed specifically for high quality research data based on features like strong gradient fields, excellent homogeneity of mg. field and excitation, high sensitivity with 64 channel head/neck coils. Simultaneous use of two scanners enables a relatively unique feature of hyper-scanning (dual fMRI). The human MR scanners are accompanied with several MR compatible electrophysiological devices for recording of high-density EEG, ECG, breathing, skin conductance, etc. The Node Candidate also offers extensive image analysis services including tailor-made software development. Besides providing access to a wide selection of equipment and analysis tools, the light microscopy unit specializes in plant in vivo imaging and techniques useful for research on live mammalian cells, mammalian germ cells, stem cells and embryos.

Contact details


Michal Mikl
Head of the Node
Representative of medical imaging within node

e-mail: michal.mikl@ceitec.muni.cz

 

Milan Ešner
Deputy head of the Node
Representative of microscopic imaging within node

e-mail: milan.esner@ceitec.muni.cz