BUT, CEITEC Publications (2016 - 2018)

CF Experimental Biophotonics

BabockÝ, J.; KŘÍŽOVÁ, A.; ŠtrbkovÁ, L.; KejÍk, L.; Ligmajer, F., HrtoŇ, M.; DvOŘÁk, P.; TÝČ, M.; ČollÁkovÁ, J.; KŘÁpek, V.; Kalousek, R.; ChmelÍk, R.; Šikola T. Quantitative 3D Phase Imaging of Plasmonic Metasurfaces. ACS Photonics, 2017. IF = 6,88

The article describes a very significant application of QPI in the field of nanophotonics, phase-altering metasurfaces. CCHM makes direct measurements of phase shifts introduced by the effect of metasurfaces possible and thus represents an important outcome of the RG photonics research, moreover published in TIER 10 journal.

Tolde, O., Gandalovičová, A., Křížová, A., Veselý, P., Chmelík,R., Rosel, D., Brábek, J. Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion, Scientific Reports, 2018. DOI:10.1038/s41598-018-30408-7. IF=4,122

World-unique quantitative-phase observations of living cells behaviour in 3D collagen have been achieved thanks to CCHM QPI that with coherence gate enabled visualization of surface membrane activity and application of dynamic phase differences (DPD) method for revealing the mass translocation processes inside the invading cell.

- Up to 5 most important technical / methodological publications (including IF) created by the members of the CzBI team at your facility

ŠtrbkovÁ, L.; Zicha D.; VeselÝ P.; ChmelÍk, R. Automated classification of cell morphology by coherence-controlled holographic microscopy. Journal of Biomedical Optics, 2017. IF = 2,367

In the last few years, the classification of cells by machine learning has become frequently used in biology. However, most of the approaches are based on morphometric features, which are not quantitative in terms of cell mass. Here we study the potential contribution of coherence-controlled holographic microscopy enabling quantitative phase imaging for the classification of cell morphologies.

GÁL, B., VESELÝ, M., ČOLLÁKOVÁ J., NEKULOVÁ, M., JŮZOVÁ, V., CHMELÍK, R., VESELÝ, P. Distinctive behaviour of head and neck squamous cell carcinoma live ex biopsy cells unveiled by coherence controlled holographic microscopy, Plos One, 2017. IF = 2,766

CCHM QPI time-lapse recordings revealed for the first time migration of seemingly static carcinoma cells in a colony as well as of individual epithelial-like cells associated with steadily increasing cell mass during migration followed with mitosis. Such data suggested an ongoing process related or identical to the epithelial-mesenchymal transition, indicated by the intermediate phenotypes of the cells in transition. We observed the loss of some epithelial markers, such as decreasing E-cadherin, ΔNp63 and KT18.