Czech-BioImaging research infrastructure has recently developed an important official document providing a comprehensive overview of Czech-BioImaging focal activities, key priorities, management structure, open-access policy and financial planning. Its purpose is to present a clear outline of long-term ambitions and the infrastructures vision as well as a complete range of services, technologies and other possibilities that Czech-BioImaging offers to its members and users. As a dynamic document, the CzBI Business plan 2021 will be updated overtime to accurately reflect its current state. Additional information regarding technical descriptions and other specialized practicalities can be found in the Technical and Implementing Annex.