Dachev, M., Bína, D., Sobotka, R., Moravcová, L., Gardian, Z., Kaftan, D., Šlouf, V., Fuciman, M., Polívka, T. & Koblížek, M. (2017) Unique double concentric ring organization of light harvesting complexes in Gemmatimonas phototrophica. PLoS Biology 15(12): e2003943. IF= 9.527
In this publication was analyzed the organization of photosynthetic (PS) complexes in the bacterium G. phototrophica, which so far is the only phototrophic representative of the bacterial phylum Gemmatimonadetes. The isolated PS complex was approximately 2 times larger than the core complex of Rhodospirillum rubrum. Electron microscopy and single particle analyses of the purified PS complexes showed that they were circular structures with an outer diameter of approximately 18 nm and a thickness of 7 nm.
Beckova, Martina; Gardian, Zdenko; Yu, Jianfeng; et al. 2017: Association of Psb28 and Psb27 Proteins with PSII-PSI Supercomplexes upon Exposure of Synechocystis sp PCC 6803 to High Light . MOLECULAR PLANT Volume: 10 Issue: 1 Pages: 62-72 IF=9,326
Formation of the multi-subunit oxygen-evolving photosystem II (PSII) complex involves a number of auxiliary protein factors. In this publication was compared the localization and possible function of two homologous PSII assembly factors, Psb28-1 and Psb28-2, from the cyanobacterium Synechocystis sp. PCC 6803. Results from single-particle electron microscopy showed the presence of large particles containing photosystem I (PSI) trimer together with two PSII monomers. The close association of PSI trimeric complexes with PSII complexes containing the Psb28 and Psb27 assembly factors supports previous hypothesis that PSI might play a protective role during PSII biogenesis.
Kostygov A., Dobáková E., Grybchuk-Ieremenko A., Váhala D., Maslov D., Votýpka J., Lukeš J., Yurchenko V. 2016: Novel trypanosomatid-bacterium association: evolution of endosymbiosis in action. mBio 7: e01985-15. iF=7.14
We describe a novel symbiotic association between a kinetoplastid protist, Novymonas esmeraldas gen. nov., sp. nov., and an intracytoplasmic bacterium, "Candidatus Pandoraea novymonadis" sp. nov., discovered as a result of a broad-scale survey of insect trypanosomatid biodiversity in Ecuador. We characterize this association by describing the morphology of both organisms (using TEM, BC) as well as their interactions, and by establishing their phylogenetic affinities. Importantly, neither partner is closely related to other known organisms previously implicated in eukaryote-bacterial symbiosis.
Tashyreva D., Prokopchuk G., Votýpka J., Yabuki A., Horák A., Lukeš J. 2018: Life cycle, ultrastructure, and phylogeny of new diplonemids and their endosymbiotic bacteria. mBio 9: e02447-17. [IF=6.689]
Authors describes two new members of the genus Diplonema (Diplonemea, Euglenozoa), Diplonema japonicum sp. nov. and Diplonema aggregatum sp. nov., based on life cycle, morphology (both the scanning and transmission electron microscopy, LEM, BC) and 18S rRNA gene sequences.
Bina D., Herbstova M., Gardian Z. et al. 2016: Novel structural aspect of the diatom thylakoid membrane: lateral segregation of photosystem I under red-enhanced illumination SCI REP 6: e25583. doi:10.1038/srep25583 IF= 4.122
Spatial segregation of photosystems in the thylakoid membrane observed in plants and in the green algae is usually considered to be absent in photoautotrophs possessing secondary plastids, such as diatoms. Contrary to this assumption, electron microscopy images in this publication show that thylakoid membranes in the chloroplast of a marine diatom, Phaeodactylum tricornutum, contain large areas occupied exclusively by a supercomplex of photosystem I (PSI) and its associated Lhcr antenna. These membrane areas, hundreds of nanometers in size, comprise hundreds of tightly packed PSI-antenna complexes while lacking other components of the photosynthetic electron transport chain.
Sytnyk M., Jakešová M., Litviňuková M., Mashkov O., Kriegner D., Stangl J., Nebesářová J., Fecher F.W., Schöfberger W., Sariciftci N.S., Schindl R., Heiss W., Głowacki E.D. (2017) Cellular interfaces with hydrogen-bonded organic semiconductor hierarchical nanocrystals Nature Communications 8: 91. [IF=12.124]
The authors described a methodology for obtaining size-controlled and shape controlled nanocrystalline hierarchical assamblies of the organic semiconducting building block quinacridone, a nontoxic magenta-coloured pigment industrially produced primarily for inks and paints. To understand the growth over time and different stages of the synthesis were imaged by scanning electron microscope (LEM, BC).
Skalický T., Dobáková E., Wheeler R.J., Tesařová M., Flegontov P., Jirsová D., Votýpka J., Yurchenko V., Ayala F., Lukeš J. (2017) Extensive flagellar remodeling during the complex life cycle of Paratrypanosoma, an early-branching trypanosomatid Proceedings of the National Academy of Sciences of the United States of America 114: 11757-11762, [IF=10,359]
Authors demonstrated that Paratrypanosoma, the most basal-branching trypanosomatid derived from free-living bodonids, assumes three different morphotypes characteristic of trypanosomatids. Electron microscopy imaging enable demonstration of Paratrypanosoma morphology, flagellum–cell attachment (similar to Leishmania) and a presence of complex cytostome architecture (similar to Trypanosoma cruzi cytostome).
Kaurov I., Vancová M., Schimanski B., Cadena L.R., Heller J., Bílý T., Potěšil D., Eichenberger C., Bruce H., Oeljeklaus S., Warscheid B., Zdráhal Z., Schneider A, Lukeš J., H. Hashimi H., 2018: The Diverged Trypanosome MICOS Complex as a Hub for Mitochondrial Cristae Shaping and Protein Import. Current Biology 28: 3393-3407, e5 [IF=9.972]
The MICOS, the mitochondrial contact site and cristae organizing system, is a conserved feature of mitochondria. With the help of colleagues from the LEM (BC), the team of scientists could directly observe the MICOS complex on mitochondrial cristae of Trypanosoma brucei and monitor the cristae morphology in cells depleted of individual TbMICOS subunits. This study demonstrates that the MICOS protein complex in trypanosomes not only helps to give christ shape, but also place protein complexes in that part of the mitochondria that is responsible for cellular respiration.
Pena Diaz C., Vancová M., Resl C., Field M., Lukeš J. (2017) A leucine aminopeptidase is involved in kinetoplast DNA segregation in Trypanosoma brucei PloS Pathogens 13: e1006310. [IF=6.608]
Trypanosomes bear a single mitochondrion with its genome (kinetoplast, or kDNA) arranged as a network of circular molecules. kDNA is essential for the Trypanosoma brucei life cycle, as it is required for proper functioning of the mitochondrion and progression through the insect vector. Using electron microscopy, authors immunolocalized leucine aminopeptidase 1, the first identified component of „the nabelschnur“, which is a structure observed during the separation of daughter kDNA networks, whose expression is required for correct kinetoplast segregation.
Nebesářová J., Wandrol P., Vancová M. 2016: Novel method of simultaneous multiple immunogold localization on resin sections in high resolution scanning electron microscopy. Nanomedicine: Nanotechnology, Biology, and Medicine 12: 105–108. [IF=5,671]
There is presented a new method of multiple immunolabeling that is suitable for a broad spectrum of biomedical applications. The general concept is to label both sides of the ultrathin section with the thickness of 70-80 nm with different antibodies conjugated to gold nanoparticles and to distinguish the labeled side by advanced imaging methods with high resolution scanning electron microscopy, such as by correlating images acquired at different energies of primary electrons using different signals.
7 - 8 October 2020, Multifunctional Centre Chateau Lednice, Czech Republic