Course description:

The Fundamentals of Light Microscopy is a basic course for students, postdocs, technicians, and other laboratory staff in biology and biomedical science who require widefield and/or confocal microscopy for their experiments. The course consists of theoretical lectures (50%) and practical hands-on session on selected microscopes and software (50%).

Overall, this course provides an introduction to the fundamentals of light microscopy, with a focus on understanding how images are formed and how acquisition choices affect resolution, contrast, and quantitative image quality. Participants will learn core principles including diffraction and the point spread function, numerical aperture and refractive index effects, sampling and digitization, detector characteristics and noise, and the conceptual differences between major imaging modalities such as widefield, confocal, super-resolution and light sheet microscopy.

A dedicated practical session will demonstrate the learned principles directly on real microscope acquisitions. Participants will gain skills needed to operate selected microscopes and experience how changing parameters influences contrast, optical sectioning, signal-to-noise ratio, and artifacts.

Finally, the workshop concludes with an introduction to basic workflows in image processing and analysis, including foundational steps for viewing, correcting, and preparing microscopy datasets for downstream quantification using open-source and commercial tools (FIJI/ImageJ, CellProfiler, ZEN, Imaris).

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