The course is designed to provide an orientation to basic and special techniques in biomedical electron microscopy in an application-oriented manner. The course will include both theoretical and practical sessions. Sample preparation under room temperature and cryo conditions will be covered, including special workflows such as immunolabeling, freeze-fracture replica, or cryo-CLEM workflow on FIB-milled lamella. Practical aspects will be explained in the lectures and prepared as a set of practical tasks for the participants. Besides basic imaging, special techniques such as (cryo)FIB-SEM, STEM tomography and EDS analysis will be demonstrated. Special emphasis will be placed on discussing how to choose the appropriate methodology for a specific scientific project.