Course description:

The Advanced Light Microscopy Methods is an advanced course for students, postdocs, technicians, and other laboratory staff in biology and biomedical science who require specialised microscopy methods for their experiments. The course consists of theoretical lectures (25%) and practical hands-on sessions on selected microscopes (50%) and image processing software (25%).

Overall, this course provides an overview of core principles behind super-resolution, FLIM-FRET and mesoscopic imaging methods, together with corresponding essential image processing steps. Participants will learn theoretical principles behind large sample imaging, super-resolution methods like Airyscan, Optical Reassignment, Structured Illumination and Single Molecule Localisation, as well as Fluorescence Lifetime and Förster Resonance Energy Transfer Microscopy.

A dedicated practical session will demonstrate the learned principles directly on real microscope acquisitions. Participants will gain skills needed to operate selected microscopes and experience how to setup advanced experimental workflow, and perform the most common troubleshooting.

The workshop concludes with an overview of related workflows in image processing and analysis utilising related software (ZEN, LAS X). Finally, the participants will receive comprehensive guidelines for advanced analysis and image visualisation in Imaris.

Practical training will be conducted on the following systems:

• Carl Zeiss LSM880 – Airyscan
• Carl Zeiss Elyra 7 – SIM/SMLM
• Leica Stellaris 8 – FALCON FLIM
• Carl Zeiss Lightsheet 7 – SPIM
• Image processing and analysis software – ZEN, LAS X, Imaris

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