The course focuses on explaining the principles of nano-second time-resolved fluorescence detection, on demonstration of different hardware realizations, on trying various applications of FLIM in biological imaging and on testing of several ways of FLIM data analysis. The aim is to uncover the richness of information hidden in multiparametric fluorescence imaging and to inspire the participants to use the easily obtainable extra contrast in their imaging applications. An important part is to make the FLIM data analysis understandable.The course will cover the most widespread FLIM realization – acquisition in time domain based on raster scanning combined with pulsed lasers and time-correlated single photon counting (TCSPC). Participants will hands-on three different systems, covering pulsed diode lasers, white-light laser source as well as two-photon excitation, SPAD, HyD and GaAsP NDD detectors and three different TCSPC systems and software.The demonstrated applications will include FLIM-FRET, environmental sensing and label free imaging (autofluorescence, SHG, THG, CARS).