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The fully distributed infrastructure of Euro-BioImaging is coordinated by a central Hub in Turku, Finland and offers its services via 33 Nodes consisting of internationally renowned imaging facilities, which are located across 14 countries and the European Molecular Biology Laboratory, EMBL. In the Czech Republic, there are currently 3 Nodes:

Advanced Light and Electron Microscopy Multi Modal Multi Sited Node

coordinated by the Institute of Molecular Genetics, Czech Acad. Sci., Prague.

Prague Node Core Facilities

Advanced Light And Electron Microscopy

The Czech Advanced Light and Electron Microscopy Multi Modal Multi Sited Node in Prague offers a wide range of state-of-the-art light and electron microscopy equipment and techniques. The Node is also active in organizing training and courses focused on theoretical and practical aspects of basic and advanced microscopy techniques. Light microscopy instrumentation includes microscopy systems that range from routine fluorescence microscopes, confocal microscopes up to high-end super-resolution microscopy systems. In the area of electron microscopy, the Node provides expertise and cutting edge equipment for a broad range of biological sample preparation and ultrastructural imaging techniques, including cryo techniques, electron tomography, and electron energy filtering.

Available technologies/expertise

Technologies:

Specialities and expertise of the Node

The Light Microscopy (LM) core facility at IMG Czech Acad. Sci. is focused on cutting-edge microscopy techniques particularly on time-gated STED microscopy. It provides strong support in image analysis and processing, more specifically in image reconstruction and deconvolution, image registration, volume reconstruction, image segmentation, tracking, colocalization analysis, analysis of photokinetic experiments, 3D visualization and mathematical modeling and simulations of biological processes.

The Electron Microscopy (EM) core facility at IMG Czech Acad. Sci. has a long expertise in the development and optimization of sample preparation techniques for electron microscopy, e.g. development of a new system for simultaneous immunolabeling of multiple molecular targets, a special technique for combination of 3D pre-embedding immunolabeling and 2D on-section immunolabeling, a statistical tool for evaluation of clustering and colocalization of immunolabeling in EM.

The Imaging core facility at BIOCEV focuses on fluorescence super-resolution imaging, functional imaging, two-photon imaging and scanning electron microscopy. The strength in image analysis is especially in fluorescence lifetime imaging data analysis and in spatio-temporal correlation techniques, for which unique data analysis modules were developed.

Image analysis and stereology is an area of expertise of the IPHYS BioImaging Facility. It provides development of new methods and SW modules (in ImageJ, Amira or Ellipse SW environment) in the field of image processing and analysis, stereology and spatial statistics.

Imaging facility of the Institute of Experimental Botany (IFIEB) focuses on microscopy in plant research. IFIEB provides high-end instrumentation and expertise for non-invasive in vivo fluorescence microscopy in high resolution. Plant-optimized vertical-stage SR microscope with root growth tracking algorithm allows long-term imaging of seedlings growing in their natural orientation.

Contact details

Node representative

Aleš Benda, Representative of Prague Node at EuroBioImaging Panel of Nodes
e-mail: ales.benda@natur.cuni.cz

Coordinator

Institute of Molecular Genetics, Czech Acad. Sci., Prague
Contact person: Hozák, Head of the Microscopy Centre of the Institute of Molecular Genetics, Czech Acad. Sci., Prague

General/Administrative contact

e-mail: info.praguenode@czech-bioimaging.cz

Advanced Light Microscopy and Medical Imaging Multi Modal Multi Sited Node

coordinated by the Masaryk University, Brno.

Brno Node Core Facilities

Advanced Light Microscopy and Medical Imaging

The Czech Advanced Light Microscopy and Medical Imaging Multi Modal Node in Brno provides open access to a wide range of imaging technologies and expertise to all scientists through a unified and coordinated logistics approach. The light microscopy and medical imaging units organize special programs for training scientists in biological and medical imaging techniques and data analysis.

Available technologies/expertise

Technologies:

Specialities and expertise of the Node

The Euro-BioImaging Node Candidate in Brno consists of two parts. The Medical Imaging part is formed by two closely collaborating facilities. One is focused on animal ultra-high field MR imaging and spectroscopy (9.4T), the other on human MR imaging (3T) accompanied with electrophysiological techniques. Together, these facilities enable translational research and offer a complex portfolio of MRI techniques including multimodal approaches (e.g. simultaneous EEG-fMRI) and human hyper-scanning (fMRI with two participants measured simultaneously in two scanners). The Advanced Light Microscopy part consist of central core facility Cellular Imaging (CELLIM), the Experimental Biophotonics Facility and several closely cooperating laboratories offering together access to equipment, training and image analysis tools. The light microscopy is further specialized in imaging of plant systems, mammalian germs cells, stem cells and embryos, and development of image analysis tools. Additionally, the Node is able to offer holographic incoherent quantitative phase imaging in correlation with laser scanning confocal microscopy.

Contact details

Node representative

Michal Mikl, Representative of Brno Node at Euro-BioImaging Panel of Nodes for Medical imaging
e-mail: michal.mikl@ceitec.muni.cz

Milan Ešner, Representative of Brno Node for Biological imaging
e-mail: milan.esner@ceitec.muni.cz

Coordinator

Masaryk University, Brno
Contact person: Michal Mikl, Head of the Multimodal and Functional Imaging Laboratory (MAFIL) at CEITEC, Brno

General/Administrative contact

e-mail: euro-bioimaging@ceitec.muni.cz

Center for Advanced Preclinical Imaging Multi Modal Single Sited Node

coordinated by Charles University, Prague

CAPI Node Core Facility

The Center for Advanced Preclinical Imaging (CAPI) at the Charles University, Prague became a Euro-BioImaging Node as a single-sited, multimodal Node covering a wide range of biomedical imaging modalities at the preclinical level. It is equipped with X-ray, CT, MRI, MPI, PET, SPECT, optical imaging, ultrasound and photoacoustic imaging instruments, all the technologies can be combined to image single animals and co-registered images can be obtained.


Available technologies/expertise

Technologies:

  • MRI/MRS or micro-MRI/MRS
  • Optical imaging (OI)
  • CT or micro-CT
  • PET/CT or micro-PET/CT
  • SPECT/CT or micro-SPECT/CT
  • US or micro-US
  • Photoacoustic Imaging (PAI)*

Specialities and expertise of the Node

CAPI is the only multimodal imaging center in the Czech Republic, offering hybrid imaging with co-registration of different modalities for single animals and some of the most innovative methods such as Magnetic Particle Imaging, High Frequency Ultrasound combined with Photoacoustic Imaging, and Spectral X-Ray Imaging.

Contact details

Node representative

Luděk Šefc, Representative of Prague Node at Euro-BioImaging Panel of Nodes
e-mail: sefc@cesnet.cz

Coordinator

Charles university, Prague
Contact person: Luděk Šefc, Head Of Department at Center for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine Charles University, Prague

General/Administrative contact

Pavla Francová, Project coordinator
e-mail: pavla.francova@lf1.cuni.cz

CAPI website

https://capi.lf1.cuni.cz/en

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